Part:BBa_K1620007:Design

Synechococcus metallothionenin A (SmtA)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 121
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.

Source

This part is an improvement of part BBa_K519010. It was synthesized by IDT as G-block and amplified by PCR using the following primers:

Fwd: 5' - GAA TTC GCG GCC GCT TCT AGA GAT GAC CTC AAC AAC GTT GGT - 3'

Rev: 5' - TAC TAG TAG CGG CCG CTG CAG TTA GCC GTG GCA GTT ACA GC - 3'

After this, the 3A assembly method was carried out.